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NaHCO3 = 30 mM
Pour 100 ml PBS (10x) into a flask
NaCl = 137 mM
magnesium is a co-factor for many enzymes including deoxyribonuclease (DNase) and various restriction enzymes
Ready to use media
Sodium bicarbonate ( Ph.Eur . , USP ) Pharma grade 1000 g Cell & Tissue Media NaHCO3 = 30 mMSodium bicarbonate is a white, crystalline powder commonly used in any laboratory. Sodium bicarbonate is used as a pH buffering agent. The compound can react with both acids and bases. Sodium bicarbonate reacts with the carboxyl groups to give a fast forming CO2 formation which has been used to test for the presence of carboxylic groups in proteins. Sodium bicarbonate has been observed to significantly decrease serum potassium levels as well as